[2019-02-20] RNA Framework v2.6.8 [major release]:
This version introduces a substantial change in the way rf-norm performs reactivity data normalization. Bases that are below a certain coverage or that have mutation rates > than a user-defined threshold, are now excluded from data normalization.
The recent advent of Next Generation Sequencing (NGS) techniques, has enabled transcriptome-scale analysis of the RNA epistructurome. Despite the establishment of several methods for querying RNA secondary structures (CIRS-seq, SHAPE-seq, Structure-seq, DMS-seq, PARS, SHAPE-MaP, DMS-MaPseq), and RNA post-transcriptional modifications (Ψ, m1A, m6A, m5C, hm5C, 2'-OMe) on a transcriptome-wide scale, no tool has been developed to date to enable the rapid analysis and interpretation of this data.
RNA Framework is a modular toolkit developed to deal with RNA structure probing and post-transcriptional modifications mapping high-throughput data.
Its main features are:
- Automatic reference transcriptome creation
- Automatic reads preprocessing (adapter clipping and trimming) and mapping
- Scoring and data normalization
- Accurate RNA folding prediction by incorporating structural probing data
For updates, please visit: http://www.rnaframework.com
For support requests, please post your questions to: https://groups.google.com/forum/#!forum/rnaframework
Danny Incarnato (dincarnato[at]rnaframework.com)
Assistant Professor @ University of Groningen
Department of Molecular Genetics
Incarnato et al., (2018) RNA Framework: an all-in-one toolkit for the analysis of RNA structures and post-transcriptional modifications (PMID: 29893890).
Incarnato et al., (2015) RNA structure framework: automated transcriptome-wide reconstruction of RNA secondary structures from high-throughput structure probing data (PMID: 26487736).
This program is free software, and can be redistribute and/or modified under the terms of the GNU General Public License as published by the Free Software Foundation, either version 3 of the License, or any later version.
Please see http://www.gnu.org/licenses/ for more information.
- Linux/Mac system
- Bowtie v1.1.2 (http://bowtie-bio.sourceforge.net/index.shtml), or
Bowtie v2.2.7 or greater (http://bowtie-bio.sourceforge.net/bowtie2/index.shtml)
- SAMTools v1.2 or greater (http://www.htslib.org/)
- BEDTools v2.0 or greater (https://github.com/arq5x/bedtools2/)
- Cutadapt v1.10 or greater (http://cutadapt.readthedocs.io/en/stable/index.html)
- ViennaRNA Package v2.2.0 or greater (http://www.tbi.univie.ac.at/RNA/)
- RNAstructure v5.6 or greater (http://rna.urmc.rochester.edu/RNAstructure.html)
- Perl v5.12 (or greater), with ithreads support
Perl non-CORE modules (http://search.cpan.org/):
- RNA (installed by the ViennaRNA package)
Clone the RNA Framework git repository:
git clone https://github.com/dincarnato/RNAFramework
This will create a "RNAFramework" folder.
To add RNA Framework executables to your PATH, simply type: